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Dark Samus joins the battle as Samus's echo fighter. With floatier movement, she's a little different from Samus--and she doesn't roll when dodging or jumping. If you look closely, you can see that her bombs and missiles look a little different, too.
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Dark Samus's on-screen appearance has her warp onto the stage from a dark portal.
Dark Samus features many different animations compared to Samus, which extends to her idle, walking, dashing, and dodge animations, as well as her taunts.
Many of Samus's two-handed animations are one-handed for Dark Samus, such as with Charge Shot and Missile.
Dark Samus's projectile attacks are visually different.
Charge Shot is mostly black and dark blue, with tendrils extending from the edges.
Dark Samus's Final Smash is also mostly black and dark blue.
Dark Samus's arm cannon splits apart and grows Phazon tendrils for some moves, such as her grab, Missiles, side smash, and Final Smash.
Dark Samus doesn't transform into the Morph Ball when rolling backwards.
Dark Samus has a different victory pose where she creates a duplicate of herself and then they merge back together, which references her "Dark Echo" ability in Metroid Prime 3: Corruption.
Many of Samus's moves, especially ones that possess flame effects (except for Missile and Bomb), have been changed into electric effects for Dark Samus.
Dark Samus is one of three playable characters who was originally an Assist Trophy in a previous game, alongside Little Mac and Isabelle.
Prior to formally being announced as a new echo fighter for Ultimate, her being a fighter in the game was hinted at with the exclusion of her Assist trophy as well as Samus having a different color for her dark costume in the game.
Dark Samus is the second character to be cloned from a female (in this case, Samus), with Daisy being the first.
Dark Samus is the second foreign-created character to be playable in Smash Bros, following Diddy Kong, and is the first character in the series that was created by an American developer; Retro Studios.
Dark Samus is the second playable female villain, the first being Wendy.
Though, Dark Samus is the first fully playable female villain due to Wendy just being an alternate costume for Bowser Jr.
Originally on the Super Smash Blog, Dark Samus was referred to with gender-neutral pronouns, despite being referred to as a female in scan data in the Metroid Prime games and in previous Smash Bros. trophies. This may have been due to her being composed entirely of a mutagenic substance known as Phazon, and her original form, Metroid Prime, was given gender-neutral pronouns before absorbing Samus's Phazon Suit and becoming Dark Samus. This error on the blog was later corrected.
Dark Samus is the only character to do a victory pose in her showcase video.
The victory pose shown in her showcase video greatly resembles Samus's pose in her official render in Ulimate.
Unlike Samus's Zero Laser, Dark Samus's Final Smash resembles a move she has actually performed in her home series: a wide, blue Phazon beam performed during the final battle of Metroid Prime 2: Echoes.
It also resembles Samus's fully charged Phazon Beam while in the Hyper Mode of her PED Suit in Metroid Prime 3: Corruption.
Just like fellow Metroid newcomer Ridley, Super Smash Bros. Ultimate marks Dark Samus's first playable appearance.
Dark Samus also marks the first time the Metroid series has a playable Metroid.
Dark Samus is currently the only Echo Fighter to be based on one of the Original 12, as the rest are based on characters debuting in Melee onwards.
Therefore, she is the only Echo Fighter based on a starter character.
According to Sakurai's weekly Famitsu column, Dark Samus was added due to her popularity overseas, while Chrom was added due to his popularity in Japan.
Dark Samus's 
