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Dark Samus joins the battle as Samus's echo fighter. With floatier movement, it's a little different from Samus--and it doesn't roll when dodging or jumping. If you look closely, you can see that its bombs and missiles look a little different, too.
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Dark Samus's on screen appearance has it warp onto the stage from a dark portal.
Dark Samus features many different animations compared to Samus, which extends to its idle, walking, dashing, and dodge animations, as well as its taunts. In addition to aesthetically hovering by default, many of its moves have slight alterations to their animations, giving it an overall "floaty and phantom-like" feel.
Many of Samus' two-handed animations are one-handed for Dark Samus, such as with Charge Shot and Missile.
Some of Dark Samus' attacks are given "corrupted" designs or effects. This can mainly be seen on its Charge Shot and its Missiles.
Dark Samus has a different victory pose, which references one of its attacks in Metroid Prime 3: Corruption.
Dark Samus doesn't transform into the Morph Ball when dodging.
Dark Samus is floatier.
Many of Samus' moves, especially ones that possess flame effects (except for Missile and Bomb), have been changed into electric effects for Dark Samus.
Dark Samus is the second playable character who was originally an Assist Trophy, the first being Little Mac.
Dark Samus is the second character to be cloned from a female, being Samus.
Dark Samus is the second foreign-created character to be playable in Smash Bros, following Diddy Kong, and is the first character in the series that was created by an American developer; Retro Studios.
Dark Samus is the second playable female villain, the first being Wendy.
Though, Dark Samus is the first fully playable female villain due to Wendy just being an alternate costume for Bowser Jr.
Despite being referred to as a female in scan data in the Metroid Prime games and in previous Smash Bros. trophies, Dark Samus is given gender-neutral pronouns in Ultimate.
Dark Samus is the only character to do a victory pose in her showcase video.
Unlike Samus' Zero Laser, Dark Samus' Final Smash resembles a move she has actually performed in her home series: a wide, blue Phazon beam performed during the final battle of Metroid Prime 2: Echoes.
It also resembles Samus' fully charged Phazon Beam while in the Hyper Mode of her PED Suit in Metroid Prime 3: Corruption.
Just like fellow Metroid newcomer Ridley, Super Smash Bros. Ultimate marks Dark Samus' first playable appearance.
Dark Samus is currently the only Echo Fighter to be based on one of the Original 12, as the rest are based on characters debuting in Melee onwards.
Dark Samus's SSB4 model was partially redesigned. 
