The following is the list of the Spirits from the Splatoon series in Super Smash Bros. Ultimate.
| Name | Type | Class | Ability | Origin game |
|---|---|---|---|---|
| Annie & Moe | Splatoon | |||
| Cap'n Cuttlefish | Splatoon | |||
| Crusty Sean | Advanced | Splatoon | ||
| Great Zapfish | Splatoon | |||
| Jelfonzo | Splatoon 2 | |||
| Judd | Splatoon | |||
| Octoling | Shield | Splatoon | ||
| Octoling Boy & Octoling Girl | Splatoon 2 | |||
| Pearl & Marina | Splatoon 2 | |||
| Spyke | Splatoon | |||
| Zapfish | Splatoon | |||
| D.J. Octavio | Splatoon |