Squid Sisters

Callie (, Aori) and Marie (, Hotaru) are supporting characters in the Splatoon franchise. Together, they make up the Squid Sisters (, Sea o' Colors) pop duo. They are Inklings, but unlike most Inklings, they have two main hair colors. Despite being called the "Squid Sisters", they are actually cousins.

Origin
In the original , Callie and Marie are a pair of pop idols who host, where they announce what stages are available for online multiplayer as well as giving information on updates and s. During Splatfests, Callie and Marie can be seen in performing their song  atop trucks, much like their Assist Trophy function, although each Squid Sister has their own truck rather than performing on the same stage, due to each of them representing one of the two Splatfest teams. These concerts can also be viewed through the use of their. Additionally, Callie and Marie appear in the fourth and fifth worlds of the game's story mode in, disguised as Agent 1 and Agent 2 respectively of the , where they replace their grandfather as the player's allies after he is kidnapped by ; in the final phase of the final boss battle against Octavio, Agents 1 and 2 reveal their identities and override Octavio's speakers with their own song, , distracting Octavio and giving Cuttlefish enough strength to break free from his ropes and begin dancing. After Octavio's defeat, the Squid Sisters host a special broadcast of Inkopolis News and decide to perform another of their songs,, which is heard as the credits roll.

In , Callie and Marie's role from the first game is taken by and  of. Marie has a major role in the story mode in, where she assists the player throughout the game, while Callie, kidnapped and brainwashed by DJ Octavio, serves as part of the final boss. However, partway through the final boss fight, Marie is able to free Callie from DJ Octavio's mind control. For the final phase of the fight, they perform together on a stage similar to the one seen in Super Smash Bros., albeit situated atop a flying truck. As of the 3.0 update of Splatoon 2, players are able to speak with Callie and Marie in Octo Canyon after completing the final battle, learning statistics about their performance in online multiplayer as well as various tidbits about the characters themselves.

In , Callie and Marie both have a major role in that game's story mode, once again assisting the player. This time they formed the "New New Squidbeak Splatoon" led by the former Agent 3 (The player character from the first game) and recruit the player to become the new Agent 3. While on a mission to rescue Cuttlefish, they had a run in with the group , who at first attempted to battle them over uncovered parts of a contraption but later decided to work with the Squid Sisters. In the end, both the Squid Sisters and Deep Cut help out the new Agent 3 during their battle against .

As an Assist Trophy
Cameras get closer and closer to their live performance, limiting the space you have to battle. Don't get too lost in the music! The Squid Sisters appear collectively as an Assist Trophy character in Ultimate. When they are summoned, they appear in the middle of the stage (teleporting if not there already), and perform a live concert. As they are performing, the camera zooms in on them, and the blast lines get smaller and smaller, making it easier for players to be KO'd, similar to the game's Sudden Death. Once the song finishes, the duo disappears, and the camera returns back to normal. They both say "Stay fresh!" in the Inkling language when summoned, which are reused voice clips from Splatoon.

During the performance, the Squid Sisters will perform one of two songs: Calamari Inkantation or Ink Me Up. Each is accompanied by a unique dance.

The Squid Sisters won't appear on stages that render characters in 2D (such as Flat Zone X and Pac-Land), stages with a constant layout change (such as Port Town Aero Dive), stages whose central area is not flat and horizontal (Mario Galaxy and Windy Hill Zone being exceptions; for Moray Towers, they appear at the lowest level) and stages with stage hazards that can engulf the majority of platforms (such as Norfair). They will not appear if a fighter is in Final Smash standby, and if a Final Smash is activated during the concert (such as by the Final Smash Meter filling), the duo will instantly disappear.

Squid Sisters can not be summoned on the following stages: Peach's Castle, Kongo Jungle, Dream Land, Rainbow Cruise, Jungle Japes, Brinstar, Yoshi's Story, Fountain of Dreams, Pokémon Stadium, Onett, Brinstar Depths, Big Blue, Delfino Plaza, Mushroomy Kingdom, WarioWare, Inc., Norfair, Halberd, Pokémon Stadium 2, Port Town Aero Dive, Castle Siege, Distant Planet, Summit, Shadow Moses Island, Luigi's Mansion, Pirate Ship, Mario Bros., Hanenbow, Green Hill Zone, 3D Land, Golden Plains, Paper Mario, Spirit Train, Dream Land GB, Unova Pokémon League, Mute City SNES, Magicant, Arena Ferox, Reset Bomb Forest, Tortimer Island, Living Room, Tomodachi Life, PictoChat 2, Mushroom Kingdom U, Mario Circuit, Skyloft, The Great Cave Offensive , Kalos Pokémon League, Flat Zone X, Gamer, Gaur Plain, , Wrecking Crew, Wuhu Island, PAC-LAND, Super Mario Maker, Midgar, New Donk City Hall, Great Plateau Tower, King of Fighters Stadium, Minecraft World, and Cloud Sea of Alrest.

As costumes
Both Squid Sisters appear as DLC Mii Costumes, with Callie being a and Marie being a  equipped with the 's Splatoon 2 design. Both were added in the Challenger Pack 6 from the Fighters Pass Vol. 2, along with Ninjara, Heihachi and Vault Boy Mii Costumes.

As a spirit
The Squid Sisters also appear as a Legend-class support spirit.

The Squid Sisters can be summoned using the cores of, , and. Also, they can be obtained by using their individual amiibo.

The Squid Sisters use an army of puppet fighters and is fought on the Moray Towers stage. During the battle, the Squid Sisters appear as a hostile Assist Trophy and the player's fighter is easy to launch, referencing the Squid Sisters' Assist Trophy effect in Ultimate where the camera zooms in on them and shrinks the blast lines, making it easy for players to KO their opponents.

Trivia

 * If the gameplay speed is lowered, the Squid Sisters will remain dancing at full speed, and will be on screen for the same amount of time. However, the screen zooming is slowed down normally, resulting in it being less effective at lower speeds.
 * Despite Splatoon featuring a playable character in the s and being a Nintendo-created and owned property, the Squid Sisters' placement in the Assist Trophy category in Training Mode and the Sound Test comes after the Assist Trophies from first and second-party universes without playable characters, and before the Assist Trophies from third-party universes.
 * The Squid Sisters were the only Assist Trophy unaffected by 's Fishing Rod glitch. If attempted, only one would spawn, and Isabelle would keep opening the Assist Trophy, but nothing would come out. This is likely due to their mechanic of zooming in the stage, and the game either putting a limit as to how far it can be zoomed in (similar to the one seen when taking a picture) or inadvertently not wanting to zoom in so far as to have it glitch and crash. However, the Devil, whose mechanic also involves moving the camera, was able to be summoned multiple times; that said, moving the camera to a different position is different from zooming in on an object.
 * Callie being a Brawler costume and Marie being a Gunner costume is a reference to their preferences in Splatoon, with Callie favoring the melee  weapons, and Marie favoring the long-ranged s.

Calamarciñas